Live Online Seminar Q & A: PCOS – Vitrification: A new method for cryopreservation

Each month we host a free, live online seminar where patients can ask the physicians of Fertility Solutions questions about infertility. The seminar for October was about ‘ Vitrification: A new method for cryopreservation ’. View the live Q & A chat about Vitrification below:

  Vitrification: A new method for cryopreservation (10/10/2012) 
6:52
Dr Ania Kowalik: 

Hello and welcome!

Wednesday October 10, 2012 6:52 Dr Ania Kowalik
6:53
Michael Lee: 

This is Michael Lee. I am the Director of Laboratories at Fertility Solutions

Wednesday October 10, 2012 6:53 Michael Lee
6:58
Michael Lee: 

If you have any questions about vitrification or egg, embryo or blastocysts cryo preservation please ask!

Wednesday October 10, 2012 6:58 Michael Lee
7:00
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Wednesday October 10, 2012 7:00 
7:01
Michael Lee: 

Hello. This is the formal definition of “Vitrification”. The main point is that there is no ice crystal formation – ice is very bad for cells!

Wednesday October 10, 2012 7:01 Michael Lee
7:13
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Wednesday October 10, 2012 7:13 
7:21
Michael Lee: 

Susan,
Thanks for the question. First, vitrification is much faster. It only takes 10 minutes or so to vitrify an embryo whereas with the old “slow freeze” methods it could take about 3 hours. Also, as you can see in my first slide – there is no ice crystal formation with vitrification which helps eggs and embryo survive the process much better

Wednesday October 10, 2012 7:21 Michael Lee
7:23
[Comment From SusanSusan: ] 

What is the difference between the “old” way of freezing embryos and vitrification?

Wednesday October 10, 2012 7:23 Susan
7:25
Michael Lee: 

Our pregnancy rates with vitrification are as good as our fresh IVF transfer rates. With the old method of embryo freezing the rates were generally about 10 – 15% less than fresh.

Wednesday October 10, 2012 7:25 Michael Lee
7:36
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Wednesday October 10, 2012 7:36 
7:40
Michael Lee: 

Vitrification isn’t actually that new – Human eggs were vitrified and produced pregnancies as early as 1999. It was first done in mouse embryos in 1985!

Wednesday October 10, 2012 7:40 Michael Lee
7:41
Michael Lee: 

If anyone would like to ask a question, please do!

Wednesday October 10, 2012 7:41 Michael Lee
7:41
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Wednesday October 10, 2012 7:41 
7:43
Michael Lee: 

This slide shows the major difference between a vitrification solution and ice crystal formation. This is what happens inside of the cells of eggs and embryos

Wednesday October 10, 2012 7:43 Michael Lee
7:45
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Wednesday October 10, 2012 7:45 
7:47
Michael Lee: 

This is what an oocyte looks like when frozen with the “slow” method. Water moves out and is replaced by cryo protectants. The danger comes when all of the water is not replaced by cryo protectant. The water will freeze and form ice crystals

Wednesday October 10, 2012 7:47 Michael Lee
7:48
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Wednesday October 10, 2012 7:48 
7:49
Michael Lee: 

With vitrification, osmotic shrinkage and the vitrification solutions cause a glass like state to occur, avoiding ice crystal formation

Wednesday October 10, 2012 7:49 Michael Lee
7:50
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Wednesday October 10, 2012 7:50 
7:50
Michael Lee: 

This is what a human egg looks like after it has been vitrified.

Wednesday October 10, 2012 7:50 Michael Lee
7:51
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Wednesday October 10, 2012 7:51 
7:52
Michael Lee: 

This is a vitrified and warmed human blastocysts that has started to hatch from the zona pellucida.

Wednesday October 10, 2012 7:52 Michael Lee
7:54
[Comment From JessicaJessica: ] 

I have a question I had my tubes tide back in November 2000, and I want to see how to to do invitro to have another child

Wednesday October 10, 2012 7:54 Jessica
7:54
Michael Lee: 

Please call our office and make an appointment with one of our physicians, they would be happy to discuss this with you

Wednesday October 10, 2012 7:54 Michael Lee
7:55
[Comment From JessicaJessica: ] 

ok at what time you will like for me to call the office ?

Wednesday October 10, 2012 7:55 Jessica
7:55
Michael Lee: 

We are open from 8AM in the morning

Wednesday October 10, 2012 7:55 Michael Lee
7:58
Michael Lee: 

Good evening. Thank you for your questions.

Wednesday October 10, 2012 7:58 Michael Lee
7:58
 

 

 
 

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